Media Peptone water

Product Description

Peptone Water can be utilized as a base for carbohydrate fermentation studies with the addition of sugar andindicators such as bromocresol purple, phenol red or bromothymol blue. 

Peptone Water is also suitable as a substratein the study of indole production. Peptone used in Peptone Water is rich in tryptophan content. Presence ofindole can be demonstrated using either Kovacs or Ehlrich reagent.Peptone Water is recommended (1,2,3) for studying the ability of an organism to ferment a specific carbohydratewhich aid in differentiation of genera and species. Peptone water is formulated as per Shread, Donovan and Lee (4).Peptone Water with pH adjusted to 8.4 is suitable for the cultivation and enrichment of Vibrio species.Peptone provides nitrogenous and carbonaceous compounds, long chain amino acids, vitamins provides essentialnutrients. Sodium chloride maintains the osmotic balance of the medium. To study the fermentationability of carbohydrates, saccharose, rhamnose, salicin are generally added in 0.5% amount separately to the basalmedium before or after sterilization. The acidity formed during fermentation can be detected by addition of phenolred indicator, which shows a colour change of the medium from red to yellow under acidic conditions. If desired,Durham's tube may be used to detect the gas production if produced.