Calbiotech Estradiol Elisa Kit

About Calbiotech Estradiol Elisa Kit

PRINCIPLE OF THE TEST
The Calbiotech, Inc E2 ELISA kit is based on the principle of Delayed competitive binding assaybetween E2 in the test specimen and E2 enzyme conjugate for a constant amount of anti-Estradiolmonoclonal antibody epitops (Biotin reagent). In the incubation, anti-E2 antibody biotin reagent, E2standards, controls, and samples are incubated for 45 minutes at room temperature (RT), then E2 enzymeconjugate is added on the top of the reaction mixture and incubation continues for 45 minutes more. Duringthe incubation, a fixed amount of HRP-labeled E2 competes with the endogenous E2 in the standard,sample, or quality control serum for a fixed number of binding sites of the specific E2 antibody. E2peroxidase conjugate immunologically bound to the well progressively decreases as the concentration ofE2 in the specimen increases. Unbound of anti-Estradiol Biotin Reagent and E2 peroxidase conjugate isthen removed and the wells are washed. Next, a solution of TMB Reagent is added and incubated at roomtemperature for 20 minutes, resulting in the development of blue color. The color development is stoppedwith the addition of stop solution, and the absorbance is measured spectrophotometrically at 450 nm. Astandard curve is obtained by plotting the concentration of the standard versus the absorbance

SUMMARY AND EXPLANATION OFTHE TEST
Estradiol E2 is the most potent natural Estrogen, produced mainly by the ovary, placenta, and in smalleramounts by the adrenal cortex, and the male testes. Estradiol is secreted into the blood stream where 98%bound to sex hormone binding globulin (SHBG). Estrogenic activity is effected via estradiol-receptorcomplexes which trigger the appropriate response at the follicles, uterus, breast, vagina, urethra,hypothalamus, pituitary and to a lesser extent the liver and skin. In non-pregnant women with normalmenstrual cycles, estradiol secretion follows a cyclic, biphasic pattern with the highest concentration foundimmediately prior to ovulation. During pregnancy, maternal serum Estradiol levels increase considerably, towell above the pre-ovulatory peak levels and high levels are sustained throughout pregnancy. SerumEstradiol measurements are a valuable index in evaluating a variety of menstrual dysfunctions such asprecocious or delayed puberty in girls and primary and secondary amenorrhea and menopause. Estradiollevels have been reported to be increased in patients with feminizing syndromes, gynaecomastia andtesticular tumors. In cases of infertility, serum Estradiol measurements are useful for monitoring inductionof ovulation following treatment. 

STORAGE AND STABILITY
1. Store the kit at 2-8C.
2. Keep microwells sealed in a dry bag with desiccants.
3. The reagents are stable until expiration of the kit.
4. Do not expose test reagents to heat, sun or strong light.