Calbiotech AMH Elisa Kit

About Calbiotech AMH Elisa Kit

PRINCIPLE OF THE TEST

The Calbiotech Inc., AMH ELISA is based on solid phase sandwich ELISA method. The samples

and conjugate reagent (anti-AMH biotin & HRP) are added to the wells coated with Streptavidin.

AMH in the patient's serum binds to the matched pair Abs, forming a sandwich complex and

simultaneously the complex is being immobilized on the plate through streptavidin-biotin

interactions. Unbound protein and HRP conjugate are washed off, through a washing step. Upon

addition of the substrate, the intensity of color is proportional to the concentration of AMH in the

samples. A standard curve is prepared by relating the color intensity to the concentration of

AMH.