BT Labs Human Papillomavirus Antibody, IgM Elisa Kit

About BT Labs Human Papillomavirus Antibody, IgM Elisa Kit

This kit is based on a Qualitative reverse phase enzyme immunoassay technique. The microtiter plate has been pre-coated with atarget antigen. Positive/Negative Controls or samples are added to the wells and incubate. Antibodies in the samples bind to theantigen on the plate. Unbound antibody is washed away during a washing step. A Horseradish Peroxidase (HRP) conjugateddetection antibody is then added and incubate. Unbound HRP is washed away during a washing step. TMB substrate is then addedand color develops. The reaction is stopped by addition of acidic stop solution and color changes into yellow that can be measured at450 nm. The OD of an unknown sample can then be compared to the OD of the positive and negative controls in order to determinethe presence of HPV Ab IgM.