AATCC BACTERIOSTASIS AGAR

About AATCC BACTERIOSTASIS AGAR

Suspend 35 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at15 lbs pressure (121C) for 15 minutes. Mix well and pour into sterile Petri plates 

Principle And Interpretation:

 AATCC Bacteriostasis Agar is used in accordance with the standard procedure (1, 2, 3). It may be used to carry stock culturesof Escherichia coli and Staphylococcus aureus . Also, it is used for the detection of antibacterial activity of fabrics.Peptic digest of animal tissue and beef extract are sources of carbon, nitrogen, vitamins and minerals. Sodium chloride providesessential ions.The test cultures of Escherichia coli and Staphylococcus aureus are grown in AATCC Bacteriostasis Broth (M221)for 24 hours. 1 ml of this culture is mixed with 150 ml of AATCC Bacteriostasis Agar and poured into the plate. After theagar solidifies, apply a circular sterile test fabric of 28.6 mm diameter onto the plate. Incubate at 35C for 18 - 24 hours andobserve the inhibition of growth around test fabric.